• info@icbcongress.com
• English
• Persian

• Login

Create Free Account

INTERNATIONAL CONGRESS OF

PharmacoGenetics

• Meta-Analysis of Gene Expression: Functional Food Potential of Iodine-Biofortified Lettuce in Caco-2 Cancer Cells

• Fatemeh Abolmashadi,¹ Mahdi Moridi Farimani,² Shayan Khalili Alashti,^3,*
  1. Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
  2. Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
  3. Epilepsy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  
  
  
• Introduction: While the iodization of salt remains the predominant strategy for producing iodine-enriched food, iodine deficiency disorders continue to pose a significant global health challenge, adversely impacting human quality of life. Iodine is essential for the production of thyroid hormones, which play a vital role in regulating metabolism and have been implicated in the process of carcinogenesis. This research seeks to utilize a bioinformatics meta-analysis to pinpoint critical genes associated with the influence of iodine-biofortified lettuce on the transcriptomic profile of the Caco-2 cancer cell line. Additionally, the identification of miRNAs may provide important insights into the molecular pathways involved in this effect.
• Methods: This research employed microarray bioinformatics to pinpoint potential indicator genes linked to Caco-2 cancer cells. The analysis focused on microarray dataset GSE71605, comparing cells treated with iodine-biofortified lettuce to untreated cells. Furthermore, pathway analysis, gene ontology (GO) enrichment, and protein-protein interaction (PPI) network analyses were performed to uncover essential biological pathways. The datasets underwent meta-analysis using bioinformatics tools to identify significant differentially expressed genes (DEGs), hub genes, and their associated miRNAs.
• Results: The study identified SV2A, NID2, and LAMA1 as key hub genes. The analysis of differentially expressed genes (DEGs) through gene ontology (GO) indicated that these genes were notably enriched in pathways related to folate biosynthesis, as well as the biosynthesis of phenylalanine, tyrosine, and tryptophan. Furthermore, several microRNAs, including hsa-miR-16-5p, hsa-miR-19a-3p, hsa-miR-19b-3p, hsa-miR-21-5p, hsa-miR-23a-3p, hsa-miR-27a-3p, hsa-miR-196a-5p, hsa-miR-34a-5p, hsa-miR-1-3p, hsa-miR-124-3p, hsa-miR-128-3p, hsa-miR-20b-5p, and members of the let-7 family, demonstrated significant interactions with the majority of the hub genes.
• Conclusion: The genetic factors identified in this study are essential for comprehending the impact of iodine-biofortified lettuce on Caco-2 cancer cells. Our findings indicate that cells can utilize iodine-biofortified plants as an alternative source of this trace element, suggesting their potential role in cancer treatment. Furthermore, these iodine-enriched lettuce varieties may be considered functional foods.
• Keywords: Bioinformatics, Functional food, Cancer, Meta-analysis