INTERNATIONAL CONGRESS OF
PharmacoGenetics
Antimetastatic activity of britannin, a sesquiterpene lactone isolated from Inula aucheriana against triple-negative breast cancer cells
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Maryam Hamzeloo-Moghadam,1 Sadegh Rajabi,2,*
1. Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran 1516745811, Iran
2. Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran 1434875451, Iran
- Introduction: Triple-negative breast cancer is the most aggressive form of breast tumors. Britannin is a sesquiterpene lactone isolated from Inula aucheriana with anticancer effects on different cancer cells. We aimed to evaluate the antimetastatic effect of britannin on the human MDA-MB-231 triple-negative breast cancer cell line.
- Methods: Approximately 5×105 MDA-MB-231 cells were seeded in each well of a 6-well cell culture plate, allowing for 60–70% confluency. Three horizontal scratches were made in different areas of each well using 200 µl sterile pipette tips, along with one vertical line for consistent photography. Cell debris was removed by washing with phosphate-buffered saline (PBS, pH ~ 7.4). Control wells were treated with normal DMEM, while test wells received 4 and 8 μM britannin. Images of the scratches were captured at 0, 9, 24, and 48 hours, and wound areas were measured using ImageJ software.
- Results: Treatment of MDA-MB-231 cells with britannin at a concentration of 4 µM for 48 h had no remarkable effect on the invasion of this cancer cell line compared with the control one. However, britannin treatment at a concentration of 8 μM for 48 considerably inhibited the invasiveness of these cancer cells compared to the controls.
- Conclusion: According to the results of this study, britannin prohibited the invasion and metastasis of human MDA-MB-231 in a concentration-dependent manner. This may suggest britannin as a natural compound for suppressing triple-negative breast cancer cell invasive properties.
- Keywords: Triple-negative breast cancer, Inula aucheriana, Britannin, Invasion, Metastasis.
