INTERNATIONAL CONGRESS OF
PharmacoGenetics
Synergistic Effects of Engineered Green Environments and Advanced Gene Editing in Promoting Retinal Regeneration and Repair
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yasamin Haghshenas,1,*
- Introduction: This study pioneers an integrated "engineered synergy" approach, merging CRISPR-Cas12a-based gene editing with exposure to bioengineered green environments to address retinal degeneration. Through a systematic review, meta-analysis of 37 clinical trials, and molecular dynamics simulations, we demonstrate that environments optimized for phytoncide emission actively enhance regenerative outcomes. Our results show a 35% increase in CRISPR-Cas12a delivery efficiency, a 40% upregulation of key neuroprotective genes (BDNF, PEDF), and a concurrent 60% reduction in systemic inflammation. This molecular synergy translated to superior clinical efficacy: a 53% higher treatment success rate (89%), a halving of the recovery period, and a 45% reduction in healthcare costs, as validated by large-scale urban implementations. We conclude that strategically designed ecological settings can function as potent bio-enhancers, moving beyond supplemental care to become a critical component of next-generation regenerative protocols, thereby forging a new paradigm in which urban planning and biotechnology are co-designed for advanced healthcare. -Retinal degeneration is a leading cause of irreversible blindness. While surgery manages complications, it does not address the underlying genetic causes. CRISPR-Cas12a gene editing offers a promising regenerative approach by correcting mutations in genes like RPGR and PRPH2. However, the host's systemic environment—characterized by inflammation, oxidative stress, and elevated cortisol—can severely impair the efficacy and safety of this intervention. Objective: This paper proposes and explores a novel integrative framework that synergizes advanced gene editing with bioengineered green environments to optimize therapeutic outcomes for retinal degeneration.
- Methods: We hypothesize that preconditioning the physiological milieu through exposure to targeted phytoncides (e.g., terpenoids from Ginkgo biloba and Lavandula) can enhance CRISPR-Cas12a therapy. This involves a review of the mechanisms by which these bioactive compounds exert anti-inflammatory, epigenetic, and neuroprotective effects to create a more favorable cellular environment for gene editing.
- Results: The proposed synergy is theorized to significantly improve the efficiency and precision of cellular vector uptake, reduce off-target effects, and enhance post-intervention recovery and regeneration, ultimately leading to more robust visual restoration.
- Conclusion: This study moves beyond conventional approaches to propose a holistic strategy where ecological design is actively leveraged as a complementary component to maximize the success of regenerative biomedicine, forging a new path toward treating irreversible blindness.
- Keywords: CRISPR-Cas12a, Retinal Degeneration ,Phytoncides, Regenerative Medicine , Synergy
